c-LEcta enzyme provides the missing link in a flavor compound synthesis

In a recent article which was published in ChemCatChem1, the ADH-collection of c-LEcta could provide the missing link to enable a two-enzyme cascade to synthesize the high value grapefruit flavor compound nootkatone from the readily available orange flavor valencene. Researcher in the group of Prof. Vlada Urlacher (University of Duesseldorf) achieved the oxidation of an allylic C-H bond to the ketone by a two-enzyme system involving a P450 and an alcohol dehydrogenase (ADH) enzyme. The ADH enzyme with the required profile was identified in the proprietary enzyme collection at c-LEcta which included wild-type enzymes from biodiversity screenings, well-studied ADHs, as well as mutants created with the c-LEcta enzyme engineering platform. Via careful optimization of the reaction conditions, the P450-ADH cascade system enabled space-time-yields for the synthesis of nootkatone that are outperforming those described in literature. This case illustrates the suitability of c-LEcta’s enzyme collection for the rapid implementation of biotransformation systems for the synthesis of aroma compounds.

1. S. Schulz, M. Girhard, S. K. Gaßmeyer, V. D. Jäger, D. Schwarze, A. Vogel, and V. Urlacher, ChemCatChem Selective Enzymatic Synthesis of the Grapefruit Flavor (+)-Nootkatone 2015, 7 601-604. (c-LEcta employees underlined)