Denarase® High Salt

For DNA Removal in Bioprocessing

Denarase® High Salt is an advanced version of the wild-type Serratia marcescens endonuclease, widely used for DNA removal in bioprocessing. Higher salt concentrations can lead to increased viral vector titers but inhibit enzyme activity. Denarase® High Salt was engineered to retain activity at higher salt concentrations and across a broader pH spectrum.
Denarase® High Salt is the optimal solution for nucleic acid removal in biomanufacturing processes that benefit from elevated salt conditions.

Main areas of application

  • Viral Vectors for Cell and Gene Therapies
  • Viral Vaccines
  • Oncolytic Viruses
  • Viscosity Reduction in Lysates
  • Sample Preparation in Electrophoresis and Chromotography
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Working principle

Denarase® High Salt was developed with c-LEcta’s proprietary enzyme technology platform ENESYZ®. A few targeted modifications on the surface of the standard Denarase®, led to a better tolerance for higher salt concentrations. As the enzyme remains its specificity for nucleic acids, Denarase® High Salt can be used in conditions where DNA and RNA are more accessible to the enzyme, which is especially beneficial at higher cell densities.

Your benefits

Denarase® High Salt efficiently removes undesired genetic material in viral vector and vaccine processes across the spectrum of commonly applied salt and pH levels.

This eliminates the need for enzyme activity-related buffer adjustments, thereby offering greater flexibility in process design. Due to its close similarity to the standard Denarase®, the existing Denarase® ELISA kit for detection of Serratia marcescens endonucleases can be used to monitor residual DENARASE® High Salt.

  • Designed for improved salt & pH tolerance

  • For highest process flexibility & efficiency

  • Compatible with Denarase® ELISA Kit

Denarase® High Salt is NOW available in two Quality Grades:

  • Denarase® High Salt for Research and Development (R&D) applications

  • Denarase® High Salt for clinical and commercial stage processes under GMP standards.

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Learn more about Denarase® High Salt

More information about the products, free samples and prices can be found here:

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Denarase® is a registered trademark of c-LEcta GmbH in the European Union (EU), United States of America (USA), United Kingdom (UK), China (CN), India (IN), South-Korea (KR) and Japan (JP).